与会Sino-German 2nd Workshop for Algae Bionergy & Nannochloropsis Synthetic Biology做特邀报告(20181127)
会议于2018.11.26-28在青岛举行,刘进与会做特邀报告“Towards Enrichment of Value-added Products in Algal Lipid Droplets by Targeted Metabolic Engineering”
与会第一届中英藻类生物技术与生物资源双边会议做特邀报告(20180919)
会议于2018.9.18-2018.9.21在武汉举行。刘进与会并与作特邀报告“Chromochloris zofingiensis, an astaxanthin-producing oleaginous alga for studying lipid metabolism and carotenogenesis”
Prof. Byeong-ryool "BJ" Jeong 来实验室做学术报告(20180822)
Engineering of photosynthesis and TFs for lipid production in industrial microalgae
报告人:Prof. Byeong-ryool "BJ" Jeong
Korea Advanced Institute of Science and Technology (KAIST)
时间:2018年8月22日(周二),下午 3:00 – 4:30
地点:工学院1号楼210会议室
主持人:刘进
报告摘要
Microalgae have emerged as excellent platforms for production biofuels and other chemicals that can replace current fossil fuels. However, they are currently in the state of "wild" and thus require extensive genetic and biological engineering for optimum performance in the production line. We are devoted to improve industrial microalgae by employing the age-old concepts of domestication process that have been used for crop plants, including mutagenesis and polyploidization. We also use state-of-the-art technologies including transcription factor (TF) engineering, gene editing, and metabolic engineering. Among these, TF engineering allowed us to improve industrial microalga Nannochloropsis salina for increased production of biomass and lipids. These strains were further tested for scale-up and continuous cultivation in PBR, revealing potentials that they can be utilized for production of biofuels and other biomaterials. In addition, we obtained a mutant (E5) that showed reduced light harvesting antenna complex (LHC) in Chlorella vulgaris. E5 grew better under high light conditions compared to WT, and molecular analyses revealed that E5 was defective in the antenna complex with improved photosynthetic efficiency and reduced non-photochemical quenching (NPQ). Genome sequencing of E5 revealed a point mutation in a gene encoding chloroplast signal recognition particle 43 (CpSRP43), well known for its function in antennal assembly in Chlamydomonas and plants. Complementation assay proved that this mutation was responsible for E5 phenotypes. We are also interested in improving photosynthetic efficiency by heterologous synthesis of chlorophyll b in N. salina that lacks any accessory chlorophylls. By expression of Chlamydomonas chlorophyllide a oxygenase (CrCAO), we created Nannochloropsis lines that produced Chlb for the first time. These lines showed improved growth and photosynthetic efficiency, and excitingly increased yield of lipids. Taken together, we have provided applicable proof of the concept that TFs and photosynthetic components can be engineered for improved production of biomass and biomaterials in industrial microalgae.
报告人简介
Prof. Byeong-ryool "BJ" Jeong is a distinguished phycologist working on algae biology and biotechnology. He received his BS (1983) and MSc (1985) from Pusan National University, South Korea (1973), and his PhD (1998) from Indiana University, USA, followed by a postdoc (1998-2004) and Research Assistant professor (2005-2011) at University of Nebraska-Lincoln, USA. BJ has been a Research professor at Korea Advanced Institute of Science and Technology (KAIST) since 2013. His group has broad research interests including 1) photosynthesis, 2) lipid metabolism, 3) genetic engineering of production strains of algae, 4) stress and pathology, etc, with many publications in top journals such as PNAS, J Biol Chem, Biotech Biofuels and Biotech Bioeng.
Prof. Arthur Grossman来实验室做学术报告(20180821)
Identifying novel proteins critical for photosynthetic function through bioinformatic analyses of the model green alga Chlamydomonas reinhardtii
报告人:Prof. Arthur Grossman
Carnegie Institution for Science and Stanford University
时间:2018年8月21日(周二),下午 3:00 – 4:30
地点:工学院1号楼210会议室
主持人:刘进
报告摘要
Photosynthesis is arguably the most important biological process on earth. There are numerous uncharacterized proteins associated with the function, biogenesis and regulation of the photosynthetic apparatus. To fill gaps in our understanding of photosynthetic processes, we assembled a bioinformatically-generated protein inventory designated the GreenCut. This suite of ~600 GreenCut proteins is conserved among photosynthetic organisms of the Green Lineage (Viridiplantae), but not present or poorly conserved in organisms that do not perform photosynthesis. The GreenCut includes many proteins that were previously shown to be directly involved in photosynthetic metabolism or that facilitated chloroplast biogenesis. However, the functions of approximately half of these proteins are still not known. We have recently characterized photosynthetic activity in several Chlamydomonas reinhardtii mutants disrupted for specific GreenCut proteins, including cgl71 and cpld49. The cgl71 mutant is altered in photosystem I activity while the cpld49 mutant is altered in cytochrome b6f activity. The mechanistic, regulatory and evolutionary implications of our results will be discussed.
报告人简介
Prof. Arthur Grossman is a distinguished biologist whose research ranges across the fields of photosynthesis, phytochemistry, plant biology, microbiology, and marine biology. He received his B.S. in Biology with Honors, from Brooklyn College (1973), and his Ph.D. from Indiana University (1978), followed by a postdoctoral training at The Rockefeller University. Arthur has been a Staff Scientist at Carnegie Institution for Science since 1982, and holds a courtesy appointment as Professor in the Department of Biology at Stanford University. He was the recipient of the Gilbert Morgan Smith Medal (National Academy of Sciences) in 2009 and the Darbaker Prize for work on microalgae (Botanical Society of America) in 2002. He is Co-Editor in Chief of Journal of Phycology, and has served on the editorial boards of major biological journals including the Annual Review of Genetics, Molecular Plant, Journal of Biological Chemistry, and Eukaryotic Cell. He has also served as organizers of many symposiums, meetings and conferences (elected co-Chair of the Gordon Research Conference on Photosynthesis in 2015, and Chair in 2017). His lab has broad research interests including 1) identifying new functions associated with photosynthetic processes, 2) mechanism(s) of coral bleaching, 3) metabolic switching upon anoxia, 4) regulation of nutrients metabolism, 5) symbiosis and photosynthetic organelle evolution, etc, with numerous publications in top journals such as Nature, Science, PNAS, and Plant Cell.
受邀访问马里兰大学环境科学中心(UMCES-IMET)并做学术报告(20180621)
受马里兰大学环境科学中心Prof Li邀请做学术报告“The Oleaginous Astaxanthin-producing Alga Chromochloris zofingiensis: Potential from Production to Model for Studying Lipid Metabolism and Carotenogenesis”(https://imet.usmd.edu/event/seminar-dr-jin-liu-peking-university)
与会18th International Conference on the Cell and Molecular Biology of Chlamydomonas(DC, USA; 20180617-20180621)
实验室成员2018春游(20180422)
实验室成员2018开工聚餐(20180226)
青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室2018年度海洋生物学鳌山青年学术论坛特邀报告(20180204)
接待墨西哥蒙特雷技术大学代表团成员参观实验室以及座谈交流 (20171206)
2017年12月6日,接待墨西哥蒙特雷技术大学代表团的Rocio Diaz de la Garza和Carmen Salinas Salazar,参观实验室以及座谈交流,达成初步合作意向
海洋大学访问交流并做学术报告 (20171202)
2017年12月3日,受邀前往上海海洋大学访问交流,做报告‘微藻油脂代谢及其应用’
与会中国海洋湖沼学会藻类学分会第十九次学术讨论会 (20171130)
访问中科院青岛能源过程研究所并做学术报告(20171031)
工学院能源系14级本科生陈秋怡参加的北京大学“本科生科研训练”项目(国家创新计划)顺利结题(2017-10-16)
邀请美国能源部大湖生物能源研究中心杨扬博士做学术报告(2017-08-16)
北京大学工学院能源与资源工程系学术报告
题目: Functional divergence of plant lipid metabolism pathways between Arabidopsis and Brachypodium
报告人:杨扬 博士, 美国能源部大湖生物能源研究中心
报告时间:2017年08月16日(周三) 10:00 am – 11:30 am
报告地点:工学院1号楼210会议室
主持人: 刘进
报告摘要:
同源基因在植物体内的生物学功能不仅取决于蛋白质序列、依赖于蛋白质序列的生化酶活以及分子间相互作用,并且和其所在的物种密切相关;即基因的生理功能存在依赖于物种的功能差异性。本报告以双子叶模式植物拟南芥和单子叶植物二穗短柄草为例,分别通过油脂代谢途径以及叶绿体膜脂合成途径中高度保守的同源基因在这这两个物种中所发挥的不同生理功能,首次详细阐明了这种依赖于物种的蛋白功能差异对脂类在高等植物发育过程中的功能至关重要。与此同时,本研究为利用合成生物学的手段,在不同作物中提高产油量提供了重要的理论依据。
报告人简介:
杨扬,美国能源部大湖生物能源研究中心助理研究员。杨扬本科和博士分别毕业于华中科技大学和北京大学。博士期间,从事模式植物拟南芥叶组织中油脂合成调控机理研究。2011年博士毕业后,赴密歇根州立大学,美国能源部大湖生物能源研究中心担任助理研究员,继续从事植物油脂代谢相关方面研究至今。研究兴趣包括植物脂类代谢、脂类在高等植物发育过程中的生理功能、合成生物学提高作物产油量等。在植物领域高水平学术期刊《The plant cell》, 《Plant physiology》,以及《Current opinion in biotechnology》等上发表学术论文多篇。
访问中科院大连化物所和大连大学并做学术报告(2017-06-19、20)
2017年6月19、20分别在中科院大连化学物理研究所和大连大学做学术报告“Microalgal Triacylglycerol: Biosynthesis, Regulation and Biotechnological Implications”
参加第二届藻类高峰论坛作大会报告(2017-05-11)
参加国投微藻中心博士后出站评审并做学术报告(2017-04-21)
2017年4月21,参加国投微藻中心担博士后出站评审并做学术报告“Triacylglycerol Biosynthesis and Regulation in Microalgae and its Biotechnological Implications”
访问暨南大学、华南理工大学、华南植物园、华南师范大学以及南昌大学并做学术报告(2017-03-27、28、29)
2017年4月27-29分别在暨南大学、华南理工大学、华南植物园、华南师范大学以及南昌大学做学术报告“Fit or Fat: The Mechanism and Biotechnological Implications of Triacylglycerol Biosynthesis in Microalgae”
邀请中国科学院植物研究所杨文强研究员做学术报告
题目:Light and dark metabolism in Chlamydomonas: Daytime can never understand the nighttime's darkness
报告人:杨文强 研究员
参加4th Asia-Oceania Algae Innovation Summit (AOAIS)(2016-09-21)
参加17th International Conference on the Cell and Molecular Biology of Chlamydomonas (Chlamy2016)(2016-07-02)
参加Bioenergy and Biorefinery Conference – Southeast Asia 2016 (Sustainable Manufcturing of Food, Fuels and Chemicals (2016.6.2)
BBCSA于2016.5.31-2016.6.1在新加坡举行。刘进与会并与专家们分享和探讨了生物能源与资源相关研究的最新进展。
邀请北京大学化学与分子工程学院特聘研究员邹鹏博士作学术报告(2016-04-28)
受邀参加The 2nd SDIC Microalgae Biotechnology Symposium做大会报告(2016-04-11)
主编英文专著出版(2016-02-24)
该书包括微藻与环境、微藻食品、微藻生物能源与微藻工程4个部分,共16个章节,结合刘进特聘研究员的多年研究经验,集结业内专家阐述了微藻生物技术的最新进展和发展展望。
http://www.coe.pku.edu.cn/research-news/4563
参会工学院与新奥能源研究院项目启动会暨新年联谊会(2016-01-24)
1月24日,北京大学工学院院长张东晓、党委书记孙智利一行12人出访新奥能源研究院,参加双方合作项目启动会暨新年联谊会。此次会议旨在增进双方了解,加强项目合作者之间的交流。刘进研究员出席参与了是次会议。
http://www.coe.pku.edu.cn/news-express/4541
刘进受邀参加国投集团微藻生物中心采购项目评标工作(2016-01-15)
刘进与会“首届中国2030年二氧化碳达峰路径高层论坛” (2015-12-27)
刘进被聘为北京高等学校高水平人才交叉培养毕业设计(创业类)支持计划北京工商大学的校外专家(2015-12-21)
上海海洋大学食品学院副院长王易芬教授和刘源副教授来工学院交流访问(2015-12-19)
参观访问中国检验检疫科学研究院(2015-11-27)
11月27日下午,刘进研究员、刘宾博士、以及暨南大学国际学院副院长吴希阳教授一行访问中国检验检疫科学研究院,魏霜专员接待,双方就食品转基因安全和检验方面做了深入的探讨交流。
参加北大工学院-新奥新绎健康研究院交流座谈会并做学术报告(2015-11-12)
http://www.coe.pku.edu.cn/news-focus/4313
台湾优质食品发展协会理事长孙宝年教授一行来工学院交流访问(2015-11-02)
2015年11月2日,台湾优质食品发展协会理事长、台湾海洋大学食品科学系讲座教授、台湾食品界领军人物孙宝年教授一行访问北京大学工学院,在燕南园60号楼进行座谈,随后参观了工学院食品与生物资源工程研究所。工学院副院长陈峰教授、刘进特聘研究员及刘宾博士接待了来宾。双方就未来在保健食品、食品安全及生物资源开发与利用等可能的合作领域深入地交换了意见。
http://www.coe.pku.edu.cn/news-focus/4293
邀请暨南大学李宏业教做学术报告(2015-10-27)
题目:海洋微藻脂类代谢及高值化利用 报告人:李宏业 教授(暨南大学生命科学技术学院,水体富营养化与赤潮防治广东省重点实验室) 主持人:刘进 特聘研究员 时间:2015年10月27日(周二)下午15:00 地点:力学楼434会议室 |
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报告内容摘要:
微藻脂类物质含量及成分直接影响着微藻在水产、医药、能源等领域的应用,是微藻资源利用中的重要指标。受限于对微藻脂质合成代谢流的调控机制和限制步骤的认知不够,相关微藻藻株改良研究的进展缓慢。我们针对海洋微藻脂质代谢开展研究,借鉴其他物种的脂质代谢的基础理论及研究成果,围绕提高微藻脂类物质合成与累积进行研究。构建了目标微藻的遗传转化体系,对微藻脂质代谢网络的途径进行解析,发掘了其中的潜在关键节点,包括调控脂质合成、降解、脂质组分等的调控节点,对相关的关键酶的功能、该基因的表达调控模式进行了深入分析,初步阐明脂质形成的分子基础,提升了对微藻脂质代谢网络的认知,并建立了微藻脂质代谢相关藻株改良的分子生物学数据库和基因资源库,为微藻高值化利用提供理论依据。
报告人简介:
李宏业教授2003年在香港大学获博士学位,2008年底加入暨南大学水生生物学国家重点学科,开始从事海洋微藻遗传及代谢机理的研究,率先建立了褐指藻等代表性藻种的高效电击遗传转化体系,在微藻脂质代谢机理方面取得了系列原创性成果。发现并阐明了微藻胞内膜脂重塑机制,解析了脂质高水平累积的来源,揭示了其中潜在关键节点在脂质累积中的独特作用及调控机理。发现了微藻氮磷营养盐吸收利用相关的一些关键酶及调控因子,初步揭示了其调控模式。成果为微藻高值化利用提供了理论基础。相关成果发表在Metabolic Engineering、Environmental Microbiology、Biotechnology for Biofuels、Algal Research、Marine Biotech等权威期刊。主持国家自然科学基金、科技部科技支撑计划课题、973项目子课题、广东省自然科学基金重大项目等10余项省部级以上项目;微藻脂质代谢研究成果获广东省第四届南粤科技创新优秀论文一等奖。
国际著名单细胞油脂专家Colin Ratledge教授应邀来访北京大学工学院食品与生物资源工程研究所(2015-10-23)
10月23日上午,国际著名单细胞油脂专家Colin Ratledge应工学院副院长陈峰教授邀请,首次赴京访问北京大学工学院食品与生物资源工程研究所,并作题为“Microorganisms as sources of valuable oils”的学术报告。陈峰教授和刘进研究员陪同Ratledge夫妇参观北京大学以及食品与生物资源工程研究所。
http://www.coe.pku.edu.cn/news-focus/4283
参观访问清华大学潘俊敏教授实验室(2015-10-12)
潘教授系长江特聘教授,国家杰出青年基金获得者,在衣藻纤毛的形成、解聚机理,运动功能、信号传导等方面的研究成果突出。
应邀于上海海洋大学做学术报告(2015-08-27)
受上海海洋大学孙铮副教授邀请做学术报告,题目为Characterization of diacylglycerolacyltransferases in Chlamydomonasreinhardtii reveals their distinct substrate specificity and role in prokaryotic and eukaryotic pathways of triacylglycerol biosynthesis。
http://www.shou.edu.cn/ce/36/c105a183862/page.htm